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Addgene

YEpURA-CUP1-HBTubiquitin
(Plasmid #26900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YEplac195-CUP1
  • Backbone size w/o insert (bp) 5891
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HBT-tagged ubiquitin
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    593

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site bamH1 (not destroyed)
  • 3′ cloning site bamH1 (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YEpURA-CUP1-HBTubiquitin was a gift from Peter Kaiser (Addgene plasmid # 26900 ; http://n2t.net/addgene:26900 ; RRID:Addgene_26900)

  • For your References section:

    A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking. Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P. Mol Cell Proteomics. 2006 Apr . 5(4):737-48. 10.1074/mcp.M500368-MCP200 PubMed 16432255
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