pFA6a-TRP1-PGAL1-HBTH
(Plasmid
#26898)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a-TRP-PGAL1
- Backbone size w/o insert (bp) 4150
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Vector typePCR tagging yeast
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHBTH tag N-terminal
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Insert Size (bp)355
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Pac1 (not destroyed)
- 3′ cloning site Asc1 (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-TRP1-PGAL1-HBTH was a gift from Peter Kaiser (Addgene plasmid # 26898 ; http://n2t.net/addgene:26898 ; RRID:Addgene_26898)
Map uploaded by the depositor.