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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNICD
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Alt nameNotch intracellular domain
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SpeciesM. musculus (mouse)
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Insert Size (bp)2500
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MutationE2299K
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GenBank IDNM_008714.3
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Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
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Tags
/ Fusion Proteins
- FLAG (N terminal on insert)
- b-globin polyA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCAG-Fwd (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-NICD was a gift from Nicholas Gaiano (Addgene plasmid # 26891 ; http://n2t.net/addgene:26891 ; RRID:Addgene_26891) -
For your References section:
Notch3 signaling promotes radial glial/progenitor character in the mammalian telencephalon. Dang L, Yoon K, Wang M, Gaiano N. Dev Neurosci. 2006;28(1-2):58-69. 10.1159/000090753 PubMed 16508304
Map uploaded by the depositor.