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Addgene

R26P-SA-EGFP-puro
(Plasmid #26890)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26890 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    R26/P
  • Backbone size w/o insert (bp) 11406
  • Vector type
    Mouse Targeting
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    jellyfish
  • Insert Size (bp)
    1736

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    EGFP-Clontech

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R26P-SA-EGFP-puro was a gift from Rudolf Jaenisch (Addgene plasmid # 26890 ; http://n2t.net/addgene:26890 ; RRID:Addgene_26890)
  • For your References section:

    Nuclear cloning of embryonal carcinoma cells. Blelloch RH, Hochedlinger K, Yamada Y, Brennan C, Kim M, Mintz B, Chin L, Jaenisch R. Proc Natl Acad Sci U S A. 2004 Sep 28. 101(39):13985-90. 10.1073/pnas.0405015101 PubMed 15306687