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Addgene

pFA6a-HTB-TRP1
(Plasmid #26877)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6aTRP1
  • Backbone size w/o insert (bp) 5000
  • Vector type
    PCR tagging yeast
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HTB tag
  • Insert Size (bp)
    358

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pac1 (not destroyed)
  • 3′ cloning site Asc1 (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-HTB-TRP1 was a gift from Peter Kaiser (Addgene plasmid # 26877 ; http://n2t.net/addgene:26877 ; RRID:Addgene_26877)

  • For your References section:

    HB tag modules for PCR-based gene tagging and tandem affinity purification in Saccharomyces cerevisiae. Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, Auer B, Huang L, Kaiser P. Yeast. 2006 Jun . 23(8):623-32. 10.1002/yea.1380 PubMed 16823883
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