CBFRE (mt) EGFP
(Plasmid #26870)

• Depositing Lab: Nicholas Gaiano
• Publication: Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Backbone

• Vector backbone: CBFRE-EGFP
• Backbone manufacturer: Gaiano Lab
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CBFRE
• Alt name: CBF1-responsive element
• Mutation: The mutated CBF1 binding site: GATCTGGTGTAAA CACGGGCTTGGAAAAAATTTATG
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CBFRE: four (mutated) CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the mutated CBF1 binding element is (GATCTGGTGTAAA
CACGGGCTTGGAAAAAATTTATG).