pHes5p-luc
(Plasmid #26869)

• Depositing Lab: Nicholas Gaiano
• Publication: Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Backbone

• Vector backbone: PGV-B
• Backbone manufacturer: Promega
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hes5 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 760
• Entrez Gene: Hes5 (a.k.a. bHLHb38)
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: Luc_N_Rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains the Hes5 promoter (-685 to +28).

C->T mutation in the center of the promoter does affect function.