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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDsRed2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4200
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHes5 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)760
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Entrez GeneHes5 (a.k.a. bHLHb38)
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Tag
/ Fusion Protein
- dsRed (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer DsRed1-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Hes5p from -685 to +28.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hes5p-DsRed was a gift from Nicholas Gaiano (Addgene plasmid # 26868 ; http://n2t.net/addgene:26868 ; RRID:Addgene_26868) -
For your References section:
Differential Notch signalling distinguishes neural stem cells from intermediate progenitors. Mizutani K, Yoon K, Dang L, Tokunaga A, Gaiano N. Nature. 2007 Sep 20. 449(7160):351-5. 10.1038/nature06090 PubMed 17721509
Map uploaded by the depositor.
