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Addgene

pQCXIP HBT-Ubiquitin
(Plasmid #26865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    250
  • Tag / Fusion Protein
    • HBT (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIP HBT-Ubiquitin was a gift from Peter Kaiser (Addgene plasmid # 26865 ; http://n2t.net/addgene:26865 ; RRID:Addgene_26865)

  • For your References section:

    Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry. Meierhofer D, Wang X, Huang L, Kaiser P. J Proteome Res. 2008 Oct . 7(10):4566-76. 10.1021/pr800468j PubMed 18781797
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