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Addgene

pcasper4 p [8-Gr66a+]
(Plasmid #26862)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSper4
  • Backbone size w/o insert (bp) 7855
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gr66 gustatory receptor
  • Alt name
    Gr66a
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    8000
  • Mutation
    Contains Gr66a and neighboring genes CG7066 and CG7188
  • Entrez Gene
    Gr66a (a.k.a. CG7189, Dmel\CG7189, Gr66A, GR66A, Gr66C1, GR66C1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcasper4 p [8-Gr66a+] was a gift from Craig Montell (Addgene plasmid # 26862 ; http://n2t.net/addgene:26862 ; RRID:Addgene_26862)

  • For your References section:

    A taste receptor required for the caffeine response in vivo. Moon SJ, Koettgen M, Jiao Y, Xu H, Montell C. Curr Biol. 2006 Sep 19. 16(18):1812-7. 10.1016/j.cub.2006.07.024 PubMed 16979558
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