ninaE-norpA
(Plasmid
#26854)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCNX
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameno receptor potential A
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Alt namenorpA
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SpeciesD. melanogaster (fly)
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MutationV963A
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Entrez GenenorpA (a.k.a. Dmel_CG3620, CG3620, CdkA, DIP2, Dmel\CG3620, MRE18, NORPA, NorpA, PI-PLC, PLC, PLC-beta, PLCbeta, anon-EST:Posey221, nofC, rof, x12, x13, x16, x24)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To express norpA under control of the ninaE promoter, full length norpA cDNA was excised from pHKX-norpA and subcloned into pCNX
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ninaE-norpA was a gift from Craig Montell (Addgene plasmid # 26854 ; http://n2t.net/addgene:26854 ; RRID:Addgene_26854) -
For your References section:
The SOCS box protein STOPS is required for phototransduction through its effects on phospholipase C. Wang T, Wang X, Xie Q, Montell C. Neuron. 2008 Jan 10. 57(1):56-68. 10.1016/j.neuron.2007.11.020 PubMed 18184564