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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYES2.0
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 7226
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Vector typeYeast Expression, Cre/Lox
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCreA recombinase
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Speciesrecoded CreA for expression in S. cerevisiae
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Insert Size (bp)1035
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MutationN/A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBF3060 was a gift from Nancy DaSilva & Suzanne Sandmeyer (Addgene plasmid # 26853 ; http://n2t.net/addgene:26853 ; RRID:Addgene_26853) -
For your References section:
A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, Hatfield GW, Da Silva NA, Sandmeyer S. Yeast. 2010 Sep 10. ():. 10.1002/yea.1824 PubMed 20936606
