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Addgene

pcDNA3.3_eGFP
(Plasmid #26822)

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Item Catalog # Description Quantity Price (USD)
Plasmid 26822 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA3.3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5'UTR-eGFP-3'UTR
  • Species
    jellyfish
  • Insert Size (bp)
    903

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer TK polyA reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.

Information regarding the UTR sequences and details regarding cloning can be found in the associated publication (http://www.cell.com/cms/attachment/609702/4870828/mmc1.pdf)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.3_eGFP was a gift from Derrick Rossi (Addgene plasmid # 26822 ; http://n2t.net/addgene:26822 ; RRID:Addgene_26822)

  • For your References section:

    Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ. Cell Stem Cell. 2010 Sep 29. ():. 10.1016/j.stem.2010.08.012 PubMed 20888316
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