pcDNA3.3_NDG
(Plasmid #26820)

• Depositing Lab: Derrick Rossi
• Publication: Warren et al Cell Stem Cell. 2010 Sep 29. ():.

Backbone

• Vector backbone: pcDNA3.3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5'UTR-NDGFP-3'UTR
• Species: jellyfish
• Insert Size (bp): 1131
• Mutation: nuclear destabilized eGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: TK polyA reverse

Resource Information

• Supplemental Documents:
  • Change to mRNA reprogramming protocol
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

NDG was constructed by attaching PEST domains and nuclear localization signals to GFP

Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.