pcDNA3.3_c-MYC
(Plasmid #26818)

• Depositing Lab: Derrick Rossi
• Publication: Warren et al Cell Stem Cell. 2010 Sep 29. ():.

Backbone

• Vector backbone: pcDNA3.3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5'UTR-c-MYC-3'UTR
• Alt name: c-myc
• Species: H. sapiens (human)
• Insert Size (bp): 1504
• Mutation: Sequence begins from second ATG; Synthetic UTR sequences added to c-MYC ORF
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: TK polyA reverse

Resource Information

• Supplemental Documents:
  • Change to mRNA reprogramming protocol
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.

Comment from lab: "with reference to NM_002467.4 sequence we have two mutations at following positions: 172 (A to G) and 174 (C to G) there is some confusion as to what the ref seq is exactly but these are the constructs that we've been using successfully."

Please note that the 5'UTR and 3'UTR sequences are synthetic and were added to the c-MYC ORF as described in Figure S1 and Table S2 of Warren et al., Cell Stem Cell. 2010. These UTR sequences are not endogenous c-MYC UTR sequences.

The 5' UTR sequence (including T7 promoter) is: TGGACCCTCGTACAGAAGCTAATACGACTCACTATAGGGAAATAAGAG
AGAAAAGAAGAGTAAGAAGAAATATAAGAGCCACCATG

The 3' UTR sequence is: GCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCT
CCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAA
GTGAGGGTCTAGAACTAGTGTCGACGC