lag-2 promoter:GFP (pJK590)
(Plasmid #26814)

• Depositing Lab: Judith Kimble
• Publication: Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 92(21):9839-42.

Backbone

• Vector backbone: pPD95.81
• Backbone manufacturer: Andrew Fire (Addgene Plasmid #1497)
• Backbone size w/o insert (bp): 4492
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: lag-2 promoter
• Species: C. elegans (nematode)
• Insert Size (bp): 3000
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13_Rev
• 3′ sequencing primer: GFP-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Quick confirmation: BamHI should result in two fragments: ~3kb and 4.5kb.

This is a lag-2 promoter::GFP fusion. Strong GFP in live worms. 3kb BamHI fragment of pJK520 is the complete 3kb 5' region of lag-2.