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pLv-HSA-uDys/eGFP
(Plasmid #26810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26810 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL-cPPT-X-PRE-SIN
  • Backbone size w/o insert (bp) 6656
  • Vector type
    Lentiviral ; lentiviral plasmid backbone from L. Naldini

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dystrophin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7233
  • Mutation
    deleted R4-R23 deleted exons 71-78
  • Entrez Gene
    DMD (a.k.a. BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85)
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site PacI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human (alpha) Skeletal Actin (HSA) promotor
Vp intron

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLv-HSA-uDys/eGFP was a gift from Jeffrey Chamberlain (Addgene plasmid # 26810 ; http://n2t.net/addgene:26810 ; RRID:Addgene_26810)

  • For your References section:

    Cell-lineage regulated myogenesis for dystrophin replacement: a novel therapeutic approach for treatment of muscular dystrophy. Kimura E, Han JJ, Li S, Fall B, Ra J, Haraguchi M, Tapscott SJ, Chamberlain JS. Hum Mol Genet. 2008 Aug 15. 17(16):2507-17. 10.1093/hmg/ddn151 PubMed 18511457
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