pcDNA3.1-2xFLAG-SREBP-2
(Plasmid #26807)

• Depositing Lab: Timothy Osborne
• Publication: Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-300.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sterol Regulatory Element Binding Protein 2
• Alt name: SREBP-2
• Alt name: SREBF2
• Alt name: SREBP2
• Species: H. sapiens (human)
• Insert Size (bp): 1440
• Mutation: L7V, G76S, K395R and I482L
• Entrez Gene: SREBF2 (a.k.a. SREBP-2, SREBP2, bHLHd2)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2x FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 18 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid expresses the truncated mature SREBP-2 protein (aa 1-482) which is targeted to the nucleus where it activates gene expression and not the full length SREBP-2 inactive precursor protein that is tethered to the endoplasmic reticulum. The plasmid was cloned similarly to the SREBP-1a and 1c constructs that are described in the publication listed below.

Please note that the SREBP-2 sequence contains L7V, G76S, K395R and I482L when compared to GenBank entry NP_004590.2. These changes may represent natural variants and have no known functional consequences. This is the exact plasmid used in the associated article listed below.