pcDNA3.1-2xFLAG-SREBP-1a
(Plasmid #26801)

• Depositing Lab: Timothy Osborne
• Publication: Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-300.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SREBP-1a
• Alt name: SREBP-1
• Alt name: SREBP-1a
• Species: H. sapiens (human)
• Insert Size (bp): 1470
• Entrez Gene: SREBF1 (a.k.a. HMD, IFAP2, SREBP1, bHLHd1)
• Tag / Fusion Protein:
  • 2xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry