Skip to main content
Addgene

Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
(Plasmid #26797)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26797 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4879
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1a-tTA/TetO-mCherry-P2A-Rs1
  • Alt name
    Rs1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8047
  • Mutation
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence.
  • Entrez Gene
    HTR4 (a.k.a. 5-HT4, 5-HT4R)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pcDNA3.2 expression vector with the majority of the CMV promoter deleted, and carrying the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1) was a gift from Edward Hsiao (Addgene plasmid # 26797 ; http://n2t.net/addgene:26797 ; RRID:Addgene_26797)
  • For your References section:

    Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Stem Cell Res Ther. 2011 Mar 4. 2(2):11. 10.1186/scrt52 PubMed 21375737