Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
(Plasmid
#26797)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.2
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4879
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEF1a-tTA/TetO-mCherry-P2A-Rs1
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Alt nameRs1
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SpeciesH. sapiens (human)
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Insert Size (bp)8047
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MutationHuman 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence.
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Entrez GeneHTR4 (a.k.a. 5-HT4, 5-HT4R)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pcDNA3.2 expression vector with the majority of the CMV promoter deleted, and carrying the EF1α-tTA/TetO-mCh-Rs1 cassette flanked by insulator sequences
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1) was a gift from Edward Hsiao (Addgene plasmid # 26797 ; http://n2t.net/addgene:26797 ; RRID:Addgene_26797) -
For your References section:
Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Stem Cell Res Ther. 2011 Mar 4. 2(2):11. 10.1186/scrt52 PubMed 21375737