pSS2 (hS100B)
(Plasmid #26774)

• Depositing Lab: Gary Shaw
• Publication: Smith et al Biochemistry. 1996 Jul 9. 35(27):8805-14.

Backbone

• Vector backbone: pSD80
• Backbone manufacturer: Stan Dunn
• Backbone size w/o insert (bp): 5696
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: S100B
• Species: H. sapiens (human)
• Insert Size (bp): 586
• Entrez Gene: S100B (a.k.a. NEF, S100, S100-B, S100beta)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13/pUC Reverse
• 3′ sequencing primer: pBAD-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few mismatches between author sequence and Addgene quality control sequence. These nucleotide differences do not alter the amino acid sequence or the function of the plasmid.