pCS2-Tm2AG
(Plasmid #26773)

• Depositing Lab: Shankar Srinivas
• Publication: Trichas et al BMC Biol. 2008 . 6():40.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TdTomato-2A-H2BGFP-SV40pA
• Alt name: membrane red
• Alt name: histone GFP
• Species: Aequorea victoria, Discosoma sp
• Insert Size (bp): 3233
• Mutation: mutation in 2A peptide that renders it non-functional (so the TdTomato and H2BGFP do not form two separate proteins, but remain fused)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: M13 rev

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Shahragim Tajbakhsh, Roger Tsien and Sean Megason

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry