pCAG-TAG
(Plasmid #26771)

• Depositing Lab: Shankar Srinivas
• Publication: Trichas et al BMC Biol. 2008 . 6():40.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAG-TdTomato-2A-H2BGFP-SV40pA
• Alt name: histone GFP
• Alt name: membrane red
• Species: Aequorea victoria and Discosoma sp
• Insert Size (bp): 4999

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: M13 forwards
• 3′ sequencing primer: T3, M13 rev

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Shahragim Tajbakhsh, Roger Tsien and Sean Megason
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry