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Addgene

pCMV-Xist-PA
(Plasmid #26760)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26760 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUHG17-1
  • Backbone size w/o insert (bp) 4240
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xist
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    15000
  • Entrez Gene
    Xist (a.k.a. A430022B11)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Xist-PA was a gift from Rudolf Jaenisch (Addgene plasmid # 26760 ; http://n2t.net/addgene:26760 ; RRID:Addgene_26760)

  • For your References section:

    A shift from reversible to irreversible X inactivation is triggered during ES cell differentiation. Wutz A, Jaenisch R. Mol Cell. 2000 Apr . 5(4):695-705. 10.1016/S1097-2765(00)80248-8 PubMed 10882105
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