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pNinaZScFv-BsaAI
(Plasmid #26759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26759 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSD
  • Backbone manufacturer
    Stan Dunn
  • Backbone size w/o insert (bp) 6278
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    inaZ, (alpha)c-myc ScFv
  • Alt name
    ice nucleation protein (INP)
  • Alt name
    (alpha) c-myc single chain antibody
  • Species
    Pseudomonas syringae
  • Insert Size (bp)
    4395

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer pBRrevBam
  • 3′ sequencing primer pBAD-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    inaZ gene was provided by Dr. Steven Lindow of UC Berkeley from plasmid pUC1813ICE.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An EcoRI fragment containing the 3600bp inaZ gene was ligated into NdeI/HindIII digested pSD
to create pNinaZ. A SmaI/EagI 795bp fragment containing the (alpha) c-myc ScFv gene was ligated into
partially digested (BsaAI) pNinaZ to generate pNinaZScFv-BsaAI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNinaZScFv-BsaAI was a gift from Greg Gloor (Addgene plasmid # 26759)

  • For your References section:

    Expression of single chain antibodies (ScFvs) for c-myc oncoprotein in recombinant Escherichia coli membranes by using the ice-nucleation protein of Pseudomonas syringae. Bassi AS, Ding DN, Gloor GB, Margaritis A. Biotechnol Prog. 2000 Jul-Aug;16(4):557-63. 10.1021/bp000053k PubMed 10933828
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