pNS2-sigmaVL
(Plasmid
#26756)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZS21-cIYFP
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Backbone manufacturerElowitz Lab
- Backbone size w/o insert (bp) 3700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCFP, mCherry, and cI-YFP
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Insert Size (bp)3700
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer mCherry-F
- 3′ sequencing primer mCherry-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNS2-sigmaVL was a gift from Michael Elowitz (Addgene plasmid # 26756 ; http://n2t.net/addgene:26756 ; RRID:Addgene_26756) -
For your References section:
Regulatory activity revealed by dynamic correlations in gene expression noise. Dunlop MJ, Cox RS, Levine JH, Murray RM, Elowitz MB. Nat Genet. 2008 Dec . 40(12):1493-8. 10.1038/ng.281 PubMed 19029898