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Addgene

pCS2+ centrin-RFP
(Plasmid #26753)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26753 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4097
  • Vector type
    reporter for basal body

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Centrin 4
  • Alt name
    cetn4
  • Species
    X. laevis (frog)
  • GenBank ID
    NP_001090133
  • Entrez Gene
    cetn4 (a.k.a. MGC130946)
  • Tag / Fusion Protein
    • monomeric RFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ centrin-RFP was a gift from Christopher Kintner (Addgene plasmid # 26753 ; http://n2t.net/addgene:26753 ; RRID:Addgene_26753)

  • For your References section:

    Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells. Park TJ, Mitchell BJ, Abitua PB, Kintner C, Wallingford JB. Nat Genet. 2008 Jul . 40(7):871-9. 10.1038/ng.104 PubMed 18552847
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