pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)
(Plasmid #26736)

• Depositing Lab: Gopal Thinakaran
• Publication: Vetrivel et al J Biol Chem. 2009 Feb 6. 284(6):3793-803.

Backbone

• Vector backbone: pcDNA3.1zeo (-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5014
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BACE
• Species: M. musculus (mouse)
• Insert Size (bp): 1504
• Mutation: Cys 474, 478, 482, and 485 mutated to Ala
• Entrez Gene: Bace1 (a.k.a. ASP2, Bace)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: BGH-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Wt Bace1 received from Nabil Seidah, IRCM, Montreal
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the F318L mutation present in the original Wt Bace1 from the Seidah lab is also present in this plasmid. The mutation is not known to affect function.