GFP-pGBD
(Plasmid #26735)

• Depositing Lab: William Bement
• Publication: Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4097
• Vector type: Mammalian Expression ; reporter for Rac

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rac
• Alt name: pGBD
• Species: X. laevis (frog)
• Mutation: Rac binding domain of rhotekin
• Entrez Gene: rac1 (a.k.a. mig5, p21-rac1, rac, tc-25)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • GBD/UtrCH pCS2+ author MCS map
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry