mRFP-wGBD
(Plasmid #26733)

• Depositing Lab: William Bement
• Publication: Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4097
• Vector type: Mammalian Expression ; reporter for Cdc42

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Neuronal Wiskott-Aldrich Syndrome protein
• Alt name: N-WASP
• Alt name: wGBD
• Species: H. sapiens (human)
• Insert Size (bp): 276
• Mutation: Cdc42 binding domain of N-WASP
• Entrez Gene: WASL (a.k.a. N-WASP, NWASP, WASPB)
• Tag / Fusion Protein:
  • mRFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • GBD/UtrCH pCS2+ author MCS map
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Cdc42-binding domain of N-WASP was fused to monomeric RFP to visualize active Cdc42