GFP-rGBD
(Plasmid #26732)

• Depositing Lab: William Bement
• Publication: Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4097
• Vector type: Mammalian Expression ; reporter for Rho

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rhotekin
• Alt name: rGBD
• Species: M. musculus (mouse)
• Insert Size (bp): 249
• Mutation: RhoA binding domain of rhotekin
• Entrez Gene: rtkn2 (a.k.a. plekhk1)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • GBD/UtrCH pCS2+ author MCS map
• A portion of this plasmid was derived from a plasmid made by: GST-rGBD construc was provided by K. Burridge (University of North Carolina at Chapel Hill)
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The RhoA-binding domain of rhotekin (rGBD) was fused to eGFP in order to visualize active RhoA