pLenti CMV rtTA3 Hygro (w785-1)
(Plasmid #26730)

• Purpose: 3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 
• Depositing Lab: Eric Campeau
• Publication: other plasmids (unpublished)

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 8669
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tetracycline repressor A3 mutant
• Alt name: rtTA3
• Insert Size (bp): 707

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: Pvu II (destroyed during cloning)
• 5′ sequencing primer: CMVforw
• 3′ sequencing primer: WPRErev

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: The rtTA3 element was a gift from Dr. Dominic Esposito (SAIC Frederick)
• Articles Citing this Plasmid:
  • 25 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be used to generate Tet-On advanced cell lines. It has been reported that it works
better than other tetracycline repressor (Markusic et al. 2005).

This plasmid has a mismatch in the first attB site. The consequences of this mismatch are not clear, however depositor successfully made 2 different cell lines with that construct.