pCS2+ cas
(Plasmid
#26729)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typexenopus/mammalian/avian/zebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecasanova
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)923
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Entrez Genesox32 (a.k.a. 10J3, 226D7, CG569, cas, cb527)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For CG569/cas probe synthesis, NotI was used to linearize the plasmid and T7 RNA polymerase to generate antisense RNA.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+ cas was a gift from Didier Stainier (Addgene plasmid # 26729 ; http://n2t.net/addgene:26729 ; RRID:Addgene_26729) -
For your References section:
casanova encodes a novel Sox-related protein necessary and sufficient for early endoderm formation in zebrafish. Kikuchi Y, Agathon A, Alexander J, Thisse C, Waldron S, Yelon D, Thisse B, Stainier DY. Genes Dev. 2001 Jun 15. 15(12):1493-505. 10.1101/gad.892301 PubMed 11410530