pEGFP-C1-hRabin8 F201A
(Plasmid #26728)

• Depositing Lab: Keith Mostov
• Publication: Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rabin8
• Alt name: Rab3IP
• Alt name: rab-3A-interacting protein isoform beta 1
• Species: H. sapiens (human)
• Mutation: F201A: GEF domain mutant
• GenBank ID: NP_783323
• Entrez Gene: RAB3IP (a.k.a. RABIN3, RABIN8)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: 3' CMV Fwd
• 3′ sequencing primer: fastbac_rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Johan Peranen provided WT Rabin8 from PMID: 12221131.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

F201A mutant in GEF domain of human Rabin8alpha.