pEGFP-C1-hRabin8 L196A
(Plasmid
#26727)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26727 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRabin8
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Alt nameRab3IP
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Alt namerab-3A-interacting protein isoform beta 1
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SpeciesH. sapiens (human)
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MutationL196A: GEF domain mutant.
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GenBank IDNP_783323
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Entrez GeneRAB3IP (a.k.a. RABIN3, RABIN8)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer 3' CMV Fwd
- 3′ sequencing primer fastbac_rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJohan Peranen (U. Helsinki). Derived from pEGFP-C1-Rabin8 from PMID: 12221131.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
L196A mutant in GEF domain.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-hRabin8 L196A was a gift from Keith Mostov (Addgene plasmid # 26727 ; http://n2t.net/addgene:26727 ; RRID:Addgene_26727) -
For your References section:
A molecular network for de novo generation of the apical surface and lumen. Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, Martin-Belmonte F, Mostov KE. Nat Cell Biol. 2010 Oct 3. ():. 10.1038/ncb2106 PubMed 20890297