GFP-Talin-L432G
(Plasmid #26725)

• Depositing Lab: Anna Huttenlocher
• Publication: Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Talin
• Species: M. musculus (mouse)
• Mutation: Changed Leucine 432 to Glycine to block ability of calpain to proteolyse talin
• Entrez Gene: Tln1 (a.k.a. Tln)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GFP-Talin L432G only differs from GFP-Talin WT in the codon comprised of base pairs 2677-2679. WT = CTT, L432G = GGT. This changes Leucine 432 to a Glycine