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Addgene

pCAG-YTB
(Plasmid #26721)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 26721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-venusYFP
  • Backbone size w/o insert (bp) 3520
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    venusYFP, TVA950, Rabies Glycoprotein
  • Alt name
    TVA
  • Alt name
    RABVgp4
  • Alt name
    EnvA
  • Species
    G. gallus (chicken); Rabies virus (strain SAD B19)
  • Insert Size (bp)
    2901
  • GenBank ID
    M31046
  • Entrez Gene
    RABVgp4 (a.k.a. RABVgp4)
  • Entrez Gene
    CD320 (a.k.a. TVA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site StuI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene sequencing result shows a CMV promoter is present. A CAG promoter was not identified as the plasmid name implies.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-YTB was a gift from Edward Callaway (Addgene plasmid # 26721 ; http://n2t.net/addgene:26721 ; RRID:Addgene_26721)

  • For your References section:

    Targeting single neuronal networks for gene expression and cell labeling in vivo. Marshel JH, Mori T, Nielsen KJ, Callaway EM. Neuron. 2010 Aug 26. 67(4):562-74. 10.1016/j.neuron.2010.08.001 PubMed 20797534
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