pBAV1K-T5-gfp
(Plasmid #26702)

• Depositing Lab: Ichiro Matsumura
• Publication: Bryksin et al PLoS One. 2010 . 5(10):e13244.

Backbone

• Vector backbone: pBAV1K
• Backbone size w/o insert (bp): 2792
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Any strain that has LacI repressor; High level of GFP expressed from the vector kills E. coli when in stationary phase.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T5lacOlacOeGFP biobrick
• Species: Synthetic construct
• Insert Size (bp): 861
• GenBank ID: HQ191434

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, XbaI (not destroyed)
• 3′ cloning site: SpeI, PstI (not destroyed)
• 5′ sequencing primer: GACGAACTCCAATTCACTGTTCCTTGC
• 3′ sequencing primer: GGAGAGCGTTCACCGACAAACAACAG

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In our experience, plasmid yields are highest when cells are harvested at late log stage.