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Addgene

VZV ORF47
(Plasmid #26698)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26698 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCGN
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ORF47
  • Species
    Varicella Zoster Virus (HHV-3)
  • Mutation
    A 3B3 epitope tag (DQRQYGDVFKGD) from the VZV gB protein is inserted at amino acid #50
  • Entrez Gene
    ORF47
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ORF47 accession no.: AF314218.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VZV ORF47 was a gift from Robert Kalejta (Addgene plasmid # 26698 ; http://n2t.net/addgene:26698 ; RRID:Addgene_26698)

  • For your References section:

    Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases. Kuny CV, Chinchilla K, Culbertson MR, Kalejta RF. PLoS Pathog. 2010 . 6(9):. 10.1371/journal.ppat.1001092 PubMed 20838604
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