Skip to main content
Addgene

KSHV ORF36 KD
(Plasmid #26696)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCGN
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ORF36
  • Species
    Kaposi's Sarcoma Associated Herpesvirus (HHV-8)
  • Mutation
    Kinase deficient mutant Lys 108 mutated to Gln
  • Entrez Gene
    ORF36
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ORF36 accession no.: AF148805

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KSHV ORF36 KD was a gift from Robert Kalejta (Addgene plasmid # 26696 ; http://n2t.net/addgene:26696 ; RRID:Addgene_26696)

  • For your References section:

    Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases. Kuny CV, Chinchilla K, Culbertson MR, Kalejta RF. PLoS Pathog. 2010 . 6(9):. 10.1371/journal.ppat.1001092 PubMed 20838604
Related items:
Commonly requested with: