pKSV7
(Plasmid #26686)

• Purpose: (Empty Backbone)
• Depositing Lab: Philip Youngman
• Publication: Smith et al Biochimie. . 74(7-8):705-11.

Backbone

• Vector backbone: pKSV7
• Backbone size (bp): 6900

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: In HB101 cells. Use 50ug/ml Amp
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

To construct pKSV7, pUCI8 [28] was partially
digested with PvulI and ligated to pBD95ts [22, 33]
linearized with Pvull (fig 1). A ligation product having
pBD95ts inserted into the PvulI site external to the
lacZ sequences was designated pKSV4; this preserves
the ability to screen for alpha-complementation when
cloning into the polylinker site. A HindlII-EcoRl
fragment containing the ble gene encoding bleomycin/
phleomycin resistance was inserted into the
HindlH-EcoRl backbone of pKSV4, replacing the
polylinker and resulting in a unique Pstl site. This Psd
site was removed by digestion with Pstl followed by
treatment with T4 DNA polymerase. The polylinker
was restored to produce pKSV7.