pET-30 Ubwt (2)
(Plasmid
#26685)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-30 Xa/Lic
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUbiquitin
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SpeciesS. cerevisiae (budding yeast)
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Tags
/ Fusion Proteins
- His (N terminal on insert)
- S-tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Same sequence as human Ub, except: S19, D24; S28
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-30 Ubwt (2) was a gift from Linda Hicke (Addgene plasmid # 26685 ; http://n2t.net/addgene:26685 ; RRID:Addgene_26685) -
For your References section:
Regulation of the RSP5 ubiquitin ligase by an intrinsic ubiquitin-binding site. French ME, Kretzmann BR, Hicke L. J Biol Chem. 2009 May 1. 284(18):12071-9. 10.1074/jbc.M901106200 PubMed 19252184