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Addgene

YCplac22-ENT2
(Plasmid #26684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCplac22
  • Backbone size w/o insert (bp) 4854
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ENT2
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ENT2 (a.k.a. YLR206W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13_puc_Rev
  • 3′ sequencing primer M13_puc_Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Yeast Replication: CEN4 ARS1
E.coli Replication: ori

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YCplac22-ENT2 was a gift from Linda Hicke (Addgene plasmid # 26684 ; http://n2t.net/addgene:26684 ; RRID:Addgene_26684)

  • For your References section:

    The function of yeast epsin and Ede1 ubiquitin-binding domains during receptor internalization. Dores MR, Schnell JD, Maldonado-Baez L, Wendland B, Hicke L. Traffic. 2010 Jan . 11(1):151-60. 10.1111/j.1600-0854.2009.01003.x PubMed 19903324
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