pCMVTNT-T7-KPNA5
(Plasmid #26681)

• Depositing Lab: Bryce Paschal
• Publication: Kelley et al BMC Cell Biol. 2010 . 11():63.

Backbone

• Vector backbone: pCMVTNT
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4050
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Karyopherin Alpha 5
• Alt name: KPNA 5
• Alt name: Importin Alpha 6
• Species: H. sapiens (human)
• Insert Size (bp): 1620
• GenBank ID: NM_002269
• Entrez Gene: KPNA5 (a.k.a. IPOA6, SRP6)
• Tag / Fusion Protein:
  • T7 (MASMTGGQQMG) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40 late poly A

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that an IS1 transposase sequence was found in the plasmid backbone during Addgene's quality control. The EcoRI digest indicates a sub-population of the stock contains this additional IS1 element. The depositor noted that this sequence should NOT affect plasmid function.