pCMVTNT-T7-KPNA3
(Plasmid #26679)

• Depositing Lab: Bryce Paschal
• Publication: Kelley et al BMC Cell Biol. 2010 . 11():63.

Backbone

• Vector backbone: pCMVTNT
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4050
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Karyopherin Alpha 3
• Alt name: KPNA3
• Alt name: Importin Alpha 4
• Species: H. sapiens (human)
• Insert Size (bp): 1566
• GenBank ID: NM_002267
• Entrez Gene: KPNA3 (a.k.a. IPOA4, SRP1, SRP1gamma, SRP4, hSRP1)
• Tag / Fusion Protein:
  • T7 (MASMTGGQQMG) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40 late poly A

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry