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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26675 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTFIIB
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SpeciesH. sapiens (human)
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Entrez GeneGTF2B (a.k.a. TF2B, TFIIB)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F, EGFP-C
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-TFIIB was a gift from Sui Huang (Addgene plasmid # 26675 ; http://n2t.net/addgene:26675 ; RRID:Addgene_26675) -
For your References section:
TBP dynamics in living human cells: constitutive association of TBP with mitotic chromosomes. Chen D, Hinkley CS, Henry RW, Huang S. Mol Biol Cell. 2002 Jan . 13(1):276-84. 10.1091/mbc.01-10-0523 PubMed 11809839