pGEM-3z/623
(Plasmid #26664)

• Purpose: (Empty Backbone)
• Depositing Lab: Jonathan Widom
• Publication: Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

Backbone

• Vector backbone: pGEM-3Z
• Backbone manufacturer: Promega
• Backbone size (bp): 2743
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HincII (unknown if destroyed)
• 3′ cloning site: HincII (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a 147 bp nucleosome positioning sequence and is referred to as clone "623".

Please note that there may be discrepancies between Addgene's sequencing results and the depositor's sequence. Potential discrepancies are not a concern according to the depositing scientist because the clones were selected for their ability to bind to histone octamer, not for their specific sequence.