pLKO.1-blast
(Plasmid #26655)

• Purpose: (Empty Backbone) 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.
• Depositing Lab: Keith Mostov
• Publication: Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Backbone

• Vector backbone: pLKO.1
• Backbone manufacturer: David Root
• Backbone size (bp): 7000
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Adapted from pLKO.1-puro (Addgene Plasmid #8453)
• Articles Citing this Plasmid:
  • 57 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Information for Cloning Grade DNA (Catalog # 26655-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here