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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5 (modified)
- Backbone size w/o insert (bp) 4107
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLatent Membrane Protein 1
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Alt nameLMP1
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SpeciesEpstein-Barr Virus
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Insert Size (bp)1420
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GenBank IDM58153
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Entrez GeneLMP-1 (a.k.a. HHV4_LMP-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p1990 SV40-LMP1 was a gift from Bill Sugden (Addgene plasmid # 26654 ; http://n2t.net/addgene:26654 ; RRID:Addgene_26654) -
For your References section:
CD40 and LMP-1 both signal from lipid rafts but LMP-1 assembles a distinct, more efficient signaling complex. Kaykas A, Worringer K, Sugden B. EMBO J. 2001 Jun 1. 20(11):2641-54. 10.1093/emboj/20.11.2641 PubMed 11387199