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Addgene

β3-integrin-YFP
(Plasmid #26653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYFP-N1
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    β3-integrin
  • Species
    H. sapiens (human)
  • Entrez Gene
    ITGB3 (a.k.a. BDPLT16, BDPLT2, BDPLT24, CD61, GP3A, GPIIIa, GT, GT2)
  • Tag / Fusion Protein
    • YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGPF-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    β3-integrin-YFP was a gift from Jonathan Jones (Addgene plasmid # 26653 ; http://n2t.net/addgene:26653 ; RRID:Addgene_26653)

  • For your References section:

    Microfilament-dependent movement of the beta3 integrin subunit within focal contacts of endothelial cells. Tsuruta D, Gonzales M, Hopkinson SB, Otey C, Khuon S, Goldman RD, Jones JC. FASEB J. 2002 Jun . 16(8):866-8. 10.1096/fj.01-0878fje PubMed 11967230
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